T InfectPlus (1000x)

T InfectPlus is a novel chemical cocktail that is formulated to enhance lentiviral and retroviral transduction efficiency in T cells. Combined with Fibronectin or Retronectin, T InfectPlus could dramatically improve the transduction efficiency in activated T cells. Protocols established with InfectPlus during process development are directly implementable for large-scale manufacturing of preclinical and clinical batches of viral vectors with the higher quality grades of T InfectPlus-premium

Example Data:

Anti-Human B cells CD19 Antibody, clone FMC63 were subclone into the second generation (with CD28 for costimulatory factor) of CAR T in LVV vector for assay testing.

T cells were activated by Dynabeads® Human T-Activator CD3/CD28 or TransAct  and tranduced by lentivirus in X-vivo 15 with 5% Human serum and IL7 (10ng/ml) +IL15 (5ng/ml).

Figure 1. Benefit of improved transduction and viability from T InfectPlus

A:Significant improvement of transduction with T InfectPlus; B: No toxicity of T InfectPlus in T cell culture; C:No negative effect on T cell expansion from T InfectPlus; D:Comparable CD4/CD8 ratio with CTR group.

Figure 2. T InfectPlus treatment  help enrich CD62L population in the CAR+ T cells

Figure 3. Better potency of CAR+ T cell after T-InfectPlus

A: T cells with T InfectPlus treatment  release higher IFNɤ after engaged with Raji cells; B: CAR+ T cells with T infectPlus treatment have better persistency in 18 days long-term killing assay.

Storage:

Store at -20° C. This product is stable for 24 months when stored as directed.

Materials Required but not Provided
Equipment:
Non-treated tissue culture plates or dishes
Electric pipetter
Pipetter
Sterile pipettes
Sterile tips with filters
Safety cabinet or clean workstation
Microscope
CO2 incubator
Microplate centrifuge
 
Reagents:
Retronectin or Fibronectin
Sterile PBS
2% bovine serum albumin (BSA Fraction V)/PBS Solution