{"title":"Lentiviral Vector Production Service","description":"\u003cp\u003e\u003cspan data-mce-fragment=\"1\"\u003eLentiviral vectors are a widely used modality for gene modification in cell therapy manufacturing processes. The innovative lentivirus production solutions from Thermo Fisher Scientific are designed to accelerate and streamline the development of gene-modified cell therapies and gene therapies.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #ff2a00;\"\u003e\u003cstrong\u003eLentiviral Packaging Workflow\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0082\/3613\/8593\/files\/hiv-lentiviral-packaging-workflow_480x480.png?v=1687844787\"\u003e\u003c\/p\u003e\n\u003ctable height=\"222\" style=\"width: 712px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 64px;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #ff2a00;\"\u003eScale\u003c\/span\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 94px;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #ff2a00;\"\u003eApplication\u003c\/span\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 109px;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #ff2a00;\"\u003eTypical Titer\u003c\/span\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 92px;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #ff2a00;\"\u003eMinimum Titer\u003c\/span\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 146px;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #ff2a00;\"\u003eVolume\u003c\/span\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #ff2a00;\"\u003eTurnaround\u003c\/span\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 198px;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #ff2a00;\"\u003ePricing\u003c\/span\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 64px;\"\u003e\n\u003ch5\u003ePilot\u003c\/h5\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"3\" style=\"width: 94px;\"\u003e\n\u003ch5\u003eCell culture\u003c\/h5\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 109px;\"\u003e\n\u003ch5\u003e\u0026gt;4x108 TU\/ml\u003c\/h5\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"2\" style=\"width: 92px;\"\u003e\n\u003ch5\u003e\u0026gt;108 TU\/ml\u003c\/h5\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 146px;\"\u003e\n\u003ch5\u003e250 ul (10x25 ul)\u003c\/h5\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"3\" style=\"width: 10px;\"\u003e\n\u003ch5\u003e8-16 days\u003c\/h5\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"3\" style=\"width: 198px;\"\u003e\n\u003ch5\u003eplease contact: info@goldenbridgen.com\u003c\/h5\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 64px;\"\u003e\n\u003ch5\u003eMedium\u003c\/h5\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 109px;\"\u003e\n\u003ch5\u003e\u0026gt;3x108 TU\/ml\u003c\/h5\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 146px;\"\u003e\n\u003ch5\u003e1 ml (10x100 ul)\u003c\/h5\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 64px;\"\u003e\n\u003ch5\u003eLarge\u003c\/h5\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 109px;\"\u003e\n\u003ch5\u003e\u0026gt;2x109 TU\/ml\u003c\/h5\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 92px;\"\u003e\n\u003ch5\u003e\u0026gt;109 TU\/ml\u003c\/h5\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 146px;\"\u003e\n\u003ch5\u003e1 ml (10x100 ul)\u003c\/h5\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","products":[{"product_id":"t-infectplus","title":"T  InfectPlus (1000x)","description":"\u003cp\u003e\u003cstrong\u003eT InfectPlus is a novel chemical cocktail that is formulated to enhance lentiviral and retroviral transduction efficiency in T cells. Combined with Fibronectin or Retronectin, T InfectPlus could dramatically improve the transduction efficiency in activated T cells. Protocols established with InfectPlus during process development are directly implementable for large-scale manufacturing of preclinical and clinical batches of viral vectors with the higher quality grades of T InfectPlus-premium\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0082\/3613\/8593\/files\/table_480x480.png?v=1610959152\" alt=\"\" data-mce-fragment=\"1\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0082\/3613\/8593\/files\/table_480x480.png?v=1610959152\"\u003e\u003c\/p\u003e\n\u003cbr\u003e\n\u003cp\u003e\u003cstrong\u003eExample Data:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAnti-Human B cells CD19 Antibody, clone FMC63 were subclone into the second generation (with CD28 for costimulatory factor) of CAR T in LVV vector for assay testing.\u003c\/p\u003e\n\u003cp\u003eT cells were activated by Dynabeads® Human T-Activator CD3\/CD28 or TransAct  and tranduced by lentivirus in X-vivo 15 with 5% Human serum and IL7 (10ng\/ml) +IL15 (5ng\/ml).\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #000000;\"\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1.\u003c\/strong\u003e Benefit of improved transduction and viability from T InfectPlus\u003c\/p\u003e\n\u003cp\u003eA:Significant improvement of transduction with T InfectPlus; B: No toxicity of T InfectPlus in T cell culture; C:No negative effect on T cell expansion from T InfectPlus; D:Comparable CD4\/CD8 ratio with CTR group.\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0082\/3613\/8593\/files\/Fig1F_480x480.jpg?v=1610958765\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2.\u003c\/strong\u003e T InfectPlus treatment  help enrich CD62L population in the CAR+ T cells\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0082\/3613\/8593\/files\/Fig2f_480x480.jpg?v=1610958788\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 3\u003c\/strong\u003e. Better potency of CAR+ T cell after T-InfectPlus\u003c\/p\u003e\n\u003cp\u003eA: T cells with T InfectPlus treatment  release higher IFNɤ after engaged with Raji cells; B: CAR+ T cells with T infectPlus treatment have better persistency in 18 days long-term killing assay.\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0082\/3613\/8593\/files\/Fig3f_480x480.jpg?v=1610958809\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eStore at -20° C. This product is stable for 24 months when stored as directed.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMaterials Required but not Provided\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eEquipment: \u003c\/strong\u003e\u003cbr\u003eNon-treated tissue culture plates or dishes\u003cbr\u003eElectric pipetter\u003cbr\u003ePipetter\u003cbr\u003eSterile pipettes\u003cbr\u003eSterile tips with filters\u003cbr\u003eSafety cabinet or clean workstation\u003cbr\u003eMicroscope\u003cbr\u003eCO2 incubator\u003cbr\u003eMicroplate centrifuge\u003cbr\u003e \u003cbr\u003e\u003cstrong\u003eReagents:\u003c\/strong\u003e\u003cbr\u003eRetronectin or Fibronectin\u003cbr\u003eSterile PBS\u003cbr\u003e2% bovine serum albumin (BSA Fraction V)\/PBS Solution\u003cbr\u003e \u003cbr\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Golden Bridgen Inc","offers":[{"title":"GBTI200","offer_id":19120917119073,"sku":"GB-TIF200","price":389.0,"currency_code":"USD","in_stock":true},{"title":"GBTI500","offer_id":19120977543265,"sku":"GB-TIF500","price":589.0,"currency_code":"USD","in_stock":true},{"title":"GBTI1000","offer_id":19120977576033,"sku":"GB-TIF1000","price":899.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0082\/3613\/8593\/products\/TinfectPlus.png?v=1590437098"},{"product_id":"293-transplus","title":"Ready-transfect-go","description":"\u003cp\u003e\u003cspan\u003eReady-transfect-go is a transfection reagent that creates biodegradable nanoparticles that permit superior transfection efficiency of plasmid DNA and siRNA into mammalian cells. Ready-transfect-go is a powerful, broadly applicable transfection reagent for effective and reproducible transfections. Transfections can be carried out entirely in the presence of serum.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"\/\/cdn.shopify.com\/s\/files\/1\/0082\/3613\/8593\/products\/new_transfect_large.jpg?v=1552720977\" alt=\"\"\u003e\u003c\/p\u003e","brand":"Golden Bridgen Inc","offers":[{"title":"1ml","offer_id":19055173435489,"sku":"1000","price":99.0,"currency_code":"USD","in_stock":true},{"title":"5ml","offer_id":19148705103969,"sku":"1001","price":399.0,"currency_code":"USD","in_stock":true},{"title":"10ml","offer_id":19148705136737,"sku":"1002","price":669.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0082\/3613\/8593\/products\/Ready-Transfect-go.png?v=1552721761"},{"product_id":"t-memory-enricher","title":"T Memory Enricher","description":"\u003cp\u003eT Memory Enricher is chemical cocktail designed to enrich the T memory population (CCR7) during the T cells expansion.  Expanded T cells treated by T Memory Enricher showed better persistence and improved efficacyin vivo (mouse Xenograft model(s)) studies. \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Golden Bridgen Inc","offers":[{"title":"GBTMC200","offer_id":19231505645665,"sku":"GBLSPTME200","price":259.0,"currency_code":"USD","in_stock":true},{"title":"GBTMC500","offer_id":19596814712929,"sku":"GBLSPTME201","price":519.0,"currency_code":"USD","in_stock":true},{"title":"GBTMC1000","offer_id":19596814745697,"sku":"GBLSPTME202","price":889.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0082\/3613\/8593\/products\/images.jpg?v=1553067580"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0082\/3613\/8593\/collections\/chemical_cocktail_600x_d3439b44-e4c2-40e7-940b-e4a542fe5225.jpg?v=1687846248","url":"https:\/\/goldenbridgen.com\/collections\/t-infectplus\/retrovirus-infection.oembed","provider":"Golden Bridgen Inc","version":"1.0","type":"link"}